lentiviral vector backbone (Addgene inc)
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Addgene inc
lentiviral vector backbone
Lentiviral Vector Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/pHR_PGK_antiCD19_synNotch_Gal4VP64+(Plasmid+%2379125)/pm41856998-208-4-8
Average 93 stars, based on 41 article reviews
Lentiviral Vector Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/pHR_PGK_antiCD19_synNotch_Gal4VP64+(Plasmid+%2379125)/pm41856998-208-4-8
Average 93 stars, based on 41 article reviews
lentiviral vector backbone - by Bioz Stars,
2026-09
93/100 stars
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Sequencing:Article Title: An ATP-gated molecular switch orchestrates human mRNA export Article Snippet: .. The full reporter sequence, consisting of the mCherry coding sequence (CDS) with a single intron containing ten boxB sites, an IRES and the GFP-puromycin resistance ORF (mCherry 1/2 -5′SS-10×boxB-IRES-GFP-Puro R -3′SS-mCherry 2/2 ), was synthesized (Genewiz) and cloned into a Synthesized:Article Title: An ATP-gated molecular switch orchestrates human mRNA export Article Snippet: .. The full reporter sequence, consisting of the mCherry coding sequence (CDS) with a single intron containing ten boxB sites, an IRES and the GFP-puromycin resistance ORF (mCherry 1/2 -5′SS-10×boxB-IRES-GFP-Puro R -3′SS-mCherry 2/2 ), was synthesized (Genewiz) and cloned into a Clone Assay:Article Title: An ATP-gated molecular switch orchestrates human mRNA export Article Snippet: .. The full reporter sequence, consisting of the mCherry coding sequence (CDS) with a single intron containing ten boxB sites, an IRES and the GFP-puromycin resistance ORF (mCherry 1/2 -5′SS-10×boxB-IRES-GFP-Puro R -3′SS-mCherry 2/2 ), was synthesized (Genewiz) and cloned into a Article Title: Structure of the human core transcription-export complex reveals a hub for multivalent interactions Article Snippet: .. For endogenous purification of THO–UAP56 from human K562 cells, the THOC1 cDNA and a C-terminal 3CAID-GFP tag were cloned into a Article Title: Structure of the human core transcription-export complex reveals a hub for multivalent interactions Article Snippet: .. For endogenous purification of THO–UAP56 from human K562 cells, the THOC1 cDNA and a C-terminal 3C-AID-GFP tag were cloned into a Article Title: mRNA recognition and packaging by the human transcription-export complex. Article Snippet: .. Wild-type ALYREF or mutant ALYREF(M-c+∆d) cDNA and a N-terminal 3×Flag tag were cloned into a Article Title: Memory cytotoxic SARS-CoV-2 spike protein-specific CD4+ T cells associate with viral control Article Snippet: .. In brief, the cDNA for the human ACE2 gene (ENSG00000130234) was cloned into a Plasmid Preparation:Article Title: An ATP-gated molecular switch orchestrates human mRNA export Article Snippet: .. The full reporter sequence, consisting of the mCherry coding sequence (CDS) with a single intron containing ten boxB sites, an IRES and the GFP-puromycin resistance ORF (mCherry 1/2 -5′SS-10×boxB-IRES-GFP-Puro R -3′SS-mCherry 2/2 ), was synthesized (Genewiz) and cloned into a Article Title: Structure of the human core transcription-export complex reveals a hub for multivalent interactions Article Snippet: .. For endogenous purification of THO–UAP56 from human K562 cells, the THOC1 cDNA and a C-terminal 3CAID-GFP tag were cloned into a Article Title: Structure of the human core transcription-export complex reveals a hub for multivalent interactions Article Snippet: .. For endogenous purification of THO–UAP56 from human K562 cells, the THOC1 cDNA and a C-terminal 3C-AID-GFP tag were cloned into a Article Title: Engineering programmable CAR and antigen pairing via drug-gated light activation. Article Snippet: The truncated CD19 (tCD19) reporter was constructed by replacing CD19CAR with tCD19 (amino acid 1-313) as amplified from Addgene #174610 with codon optimization for human cell line expression (Integrated DNA Technologies, CA). .. For lentivirus production, the Article Title: mRNA recognition and packaging by the human transcription-export complex. Article Snippet: .. Wild-type ALYREF or mutant ALYREF(M-c+∆d) cDNA and a N-terminal 3×Flag tag were cloned into a Article Title: Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems Article Snippet: .. The following plasmid backbones were used in this study: lentiCRISPR v2 (lentiv2, Addgene #52961): a Article Title: Memory cytotoxic SARS-CoV-2 spike protein-specific CD4+ T cells associate with viral control Article Snippet: .. In brief, the cDNA for the human ACE2 gene (ENSG00000130234) was cloned into a Purification:Article Title: Structure of the human core transcription-export complex reveals a hub for multivalent interactions Article Snippet: .. For endogenous purification of THO–UAP56 from human K562 cells, the THOC1 cDNA and a C-terminal 3CAID-GFP tag were cloned into a Article Title: Structure of the human core transcription-export complex reveals a hub for multivalent interactions Article Snippet: .. For endogenous purification of THO–UAP56 from human K562 cells, the THOC1 cDNA and a C-terminal 3C-AID-GFP tag were cloned into a Mutagenesis:Article Title: mRNA recognition and packaging by the human transcription-export complex. Article Snippet: .. Wild-type ALYREF or mutant ALYREF(M-c+∆d) cDNA and a N-terminal 3×Flag tag were cloned into a Expressing:Article Title: Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems Article Snippet: .. The following plasmid backbones were used in this study: lentiCRISPR v2 (lentiv2, Addgene #52961): a |
![( A ) Inducible gRNA barcode design: A spacer-blocking hairpin structure is engineered to include a 15-base semirandom genetic barcode within the hairpin loop. In addition, an 8A8G poly(A) tail is appended at the 3′ end of the barcoding gRNA. ( B ) Oligo-CALL system constructs: (i) Dual gRNA barcoding construct: two distinct barcoding gRNA, each driven by human or mouse U6 promoters, separately, with Thy1.1 as a constitutive selection marker. (ii) The green fluorescent protein (GFP) reporter construct: contains three tandem repeats of the barcoding gRNA target site upstream of a miniCMV (minimal cytomegalovirus) promoter controlling GFP expression; blue fluorescent protein (BFP) serves as a selection marker. (iii) CRISPRa construct: constitutively expresses dCas9-VPR [deactivated Cas9 fused to a transcriptional activator VPR (VP64, p65, and Rta)] and the red fluorescent protein (RFP; Cherry variant). All constructs use the <t>lentiviral</t> backbone to facilitate efficient cell transduction. hU6, human U6; mU6, mouse U6; EF1α, elongation factor-1 alpha promoter. ( C ) GFP activation with Oligo-CALL system: In the default state, a self-folding hairpin within the gRNA spacer prevents CRISPRa from activating GFP expression. Introduction of BC-ASOs disrupts this hairpin, exposing the gRNA spacer and enabling robust GFP expression. Representative fluorescence images show strong GFP induction following BC-ASO transfection, in contrast to minimal activation in cells receiving no ASO or mismatched ASOs (mis ASOs). ( D to F ) Flow cytometry analyses of GFP activation in Oligo-CALL–labeled cells: H358 cells bearing BC1 barcodes were transfected with ASOs against BC1. Flow cytometry shows the percentage of GFP + cells (D and E) and the mean fluorescent intensity (F) across the overall population (48 hours post-ASO transfection; n = 2 biological replicates). MFI, mean fluorescence intensity. Cont., control.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4195/pmc12594195/pmc12594195__sciadv.adw9990-f1.jpg)